MicroED Imaging Center Workshop

December 11-14, 2022

MicroED on a globular protein
By MEDIC
1 min read

The Gonen laboratory will hold the 7th MicroED course in 2022. The course will be held in person December 11–14, 2022 at UCLA. The course is organized by Tamir Gonen (UCLA) and Brent Nannenga (ASU).

Topics covered

  • Sample preparation methodologies for proteins and small molecules
  • MicroED data collection
  • Camera performance
  • Cryo-FIB-milling procedures for MicroED
  • Data analysis software, structure solution, and refinement

Tentative schedule

Sunday Dec. 11th 2022

5:00 PM: Welcome Dinner

Monday Dec. 12th 2022

  • 8:30 AM – 9:00 AM: Breakfast
  • 9:00 AM – 9:20 AM: Introduction to MicroED and welcoming notes
  • 9:20 AM – 9:40 AM: Sample preparation
  • 9:40 AM – 10:00 AM: FIB milling for MicroED
  • 10:00 AM – 10:30 AM: Break
  • 10:30 AM – 11:00 AM: MicroED data collection with EPUD
  • 11:00 AM – 12:00 PM: Installation and testing of software for workshop
  • 12:00 PM – 1:00 PM: Lunch
  • 1:00 PM – 5:00 PM: Practical sessions I
  • 5:00 PM: Dinner

Tuesday Dec. 13th 2022

  • 8:30 AM – 9:00 AM: Breakfast
  • 9:00 AM – 10:00 AM: Archiving, structure deposition, and validation
  • 10:00 AM – 10:30 AM: Cameras for MicroED
  • 10:30 AM – 11:00 AM: Break
  • 11:00 AM – 11:30 AM: Equipment for MicroED
  • 11:30 AM – 12:00 PM: Data processing and structure determination of small molecules
  • 12:00 PM – 1:00 PM: Lunch
  • 1:00 PM – 5:00 PM: Practical sessions II
  • 5:00 PM: Dinner

Wednesday Dec. 14th 2022

  • 8:30 AM – 9:00 AM: Breakfast
  • 9:00 AM – 9:30 AM: Automated data collection for MicroED
  • 9:30 AM – 10:00 AM: Chemical crystallography with MicroED
  • 10:00 AM – 10:30 AM: MicroED with GPCRs
  • 10:30 AM – 11:00 AM: Enzyme structure determination by MicroED
  • 11:00 AM – 12:00 PM: Open discussion
  • 12:00 PM – 1:00 PM: Lunch
  • 1:00 PM – 5:00 PM: Practical sessions III (Sample preparation, data collection and processing)
  • 5:00 PM: Dinner and closing

Organizers

MicroED Imaging Center (MEDIC) at UCLA

Instructors

Tamir Gonen (UCLA), Brent Nannenga (ASU), Johan Hattne (UCLA), Michael Martynowycz (UCLA), Max Clabbers (UCLA), Callie Saeher (UCLA), Emma Danelius (UCLA), Anna Shiriaeva (UCLA), and Natalie Young (Thermo Fisher).

Cost

The cost for academic attendees is $500. The cost for attendees outside academia is $1,500. The cost of the workshop covers meals and other expenses associated with the course.

Application

This course is not accepting new applications.