Publications

Referencing us

MEDIC must be acknowledged in all publications that resulted from either collaboration with the Center or with individual personnel of the Center or utilized services of the Center. The NIH grants funding the MEDIC project are P41GM136508 (2020-2025) and RM1GM158451 (2025-2030).

As in all scientific publications, co-authorship for a member of the Scientific Staff is warranted when an individual has made a significant contribution to the scientific work being described.

Data policy

All data collected for structural purposes will be made publicly available upon publication. Structures will be deposited in the protein structure data bank (PDB) and the electron microscopy data bank (EMDB) prior to publication. All primary data associated with a structure will be made available to the community upon request.

Sharing of materials

All materials, plasmids, peptides, and protein fragments will be made available to the research community in accordance with NIH guidelines and in coordination with the UCLA technology development group (TDG) governing material transfer agreements (MTA).

Sharing of software and algorithms

All software and programs used for analysis or structure determination will be made available either as pseudocode or as standalone scripts/programs in accordance with NIH guidelines and in coordination with the UCLA technology development group (TDG) governing intellectual property dissemination and materials transfer agreements (MTA).

Publication list

2026

Wieske, Lianne H. E.; Bu, Guanhong; Erdélyi, Máté; Kihlberg, Jan; Gonen, Tamir; Rova Danelius, Emma
Conformat io nal Traj e cto ry of the M o le c u l a r C ha m el e o n G r azo p revir from Form u l atio n to T a rge t -B o u n d
In: Chemistry, vol. 32, no. 8, pp. e02256, 2026, ISSN: 0947-6539.

Lin, Jieye; Paz, Orel; Unge, Johan; Gonen, Tamir
Rapid Structural Analysis of Natural Products Using MicroED
In: Small, pp. e11875, 2026, ISSN: 1613-6810.

Lin, Jieye; Paz, Orel; Unge, Johan; Gonen, Tamir
Discovery of Oxyacanthine Dihydrochloride Monohydrate Polymorphs from Obfuscated Samples by Microcrystal Electron Diffraction
In: ChemMedChem, vol. 21, no. 1, pp. e202500601, 2026, ISSN: 1860-7179.

Knudson, Isaac J.; Dover, Taylor L.; Dilworth, Diondra A.; Paloutzian, Cameron; Paz, Orel; Lin, Jieye; Cho, Hannah; Gonen, Tamir; Schepartz, Alanna; Miller, Scott J.
Chemical and ribosomal synthesis of atropisomeric and macrocyclic peptides with embedded quinolines
In: Nat Chem, vol. 18, pp. 61–72, 2026, ISSN: 1755-4330.

2025

Nannenga, Brent L.; Gonen, Tamir
The 2024 challenges in structural biology summit
In: Struct Dyn, pp. 040901, 2025, ISSN: 2329-7778.

Lin, Jieye; Paz, Orel; Unge, Johan; Gonen, Tamir
Discovery of oxyacanthine dihydrochloride monohydrate polymorphs from obfuscated samples by MicroED
In: bioRxiv, 2025.

Unge, Johan.; Nannenga, Brent L.; Oliver, Allen G.; Gonen, Tamir
Standards for MicroED
In: Acta Crystallogr C Struct Chem, vol. 81, no. 7, pp. 376–390, 2025, ISSN: 2053-2296.

Gallenito, Marc J.; Clabbers, Max T. B.; Lin, Jieye; Hattne, Johan; Gonen, Tamir
High-Throughput MicroED for Probing Ion Channel Dynamics
In: Adv Sci, vol. 12, no. 30, pp. e04881, 2025, ISSN: 2198-3844.

Clabbers, Max T. B.; Gonen, Tamir
Recovering high-resolution information using energy filtering in MicroED
In: Struct Dyn, vol. 12, no. 3, pp. 034702, 2025, ISSN: 2329-7778.

Ruma, Yasmeen, N.; Nannenga, Brent L.; Gonen, Tamir
Unraveling atomic complexity from frozen samples
In: Struct Dyn, vol. 12, no. 2, pp. 020901, 2025, ISSN: 2329-7778.

Clabbers, Max T. B.; Gonen, Tamir
Recovering high-resolution information using energy filtering in MicroED
In: bioRxiv, 2025.

Nicolas, William J.; Shiriaeva, Anna; Martynowycz, Michael W.; Grey, Angus C.; Ruma, Yasmeen N.; Donaldson, Paul J.; Gonen, Tamir
Structure of the lens MP20 mediated adhesive junction
In: Nat Commun, vol. 16, pp. 2977, 2025, ISSN: 2041-1723.

Lin, Jieye; Gallenito, Marc J.; Hattne, Johan; Gonen, Tamir
Ligand Screening and Discovery using Cocktail Soaking and Automated MicroED
In: ChemMedChem, vol. 20, no. 12, pp. e2500156, 2025, ISSN: 1860-7179.

Lin, Jieye; Unge, Johan; Gonen, Tamir
MicroED Structures of Fluticasone Furoate and Fluticasone Propionate Provide New Insights into Their Function
In: Cryst Growth Des, vol. 25, no. 5, pp. 1588–1596, 2025. ISSN: 1528-7483.

Clabbers, Max T. B.; Hattne, Johan; Martynowycz, Michael W.; Gonen, Tamir
Characterization of energy filtering slit widths for MicroED data collection
In: bioRxiv, 2025.

Clabbers, Max T. B.; Hattne, Johan; Martynowycz, Michael W.; Gonen, Tamir
Energy filtering enables macromolecular MicroED data at sub-atomic resolution
In: Nat Commun, vol. 16, pp. 2247, 2025, ISSN: 2041-1723.

Lin, Jieye; Gallenito, Marc J.; Hattne, Johan; Gonen, Tamir
Ligand Screening and Discovery using Cocktail Soaking and Automated MicroED
In: bioRxiv, 2025.

Nicolas, William J.; Gillman, Cody; Weaver, Sara J.; Clabbers, Max T. B.; Shiriaeva, Anna; Sae Her, Ampon; Martynowycz, Michael W.; Gonen, Tamir
Comprehensive microcrystal electron diffraction sample preparation for cryo-EM
In: Nat Protoc, vol. 20, pp. 1275–1309, 2025, ISSN: 1750-2799.

Janilkarn-Urena, Isis; Tse, Amanda; Lin, Jieye; Tafolla-Aguirre, Bliss; Idrissova, Alina; Zhang, Mindy; Skinner, Samantha G; Mostowfi, Nader; Kim, Jinah; Kalapatapu, Nikhila; Chang, Xinmin; Efthymiou, Christina; Williams, Christopher K; Magaki, Shino D; Vinters, Harry V; Gonen, Tamir; Ahmed, S Kaleem; Gukasyan, Hovhannes J; Davies, Daryl L; Seidler, Paul M
Improving the solubility of pseudo-hydrophobic chemicals through co-crystal formulation
In: PNAS Nexus, vol. 4, no. 1, 2024, ISSN: 2752-6542.

2024

Ruma, Yasmeen N.; Bu, Guanhong; Hattne, Johan; Gonen, Tamir
MicroED structure of the C11 cysteine protease clostripain
In: Journal of Structural Biology: X, vol. 10, pp. 100107, 2024, ISSN: 2590-1524.

Liu, Xiaoyu; Xia, Xian; Martynowycz, Michael W.; Gonen, Tamir; Zhou, Z. Hong
Molecular sociology of virus-induced cellular condensates supporting reovirus assembly and replication
In: Nat Commun, vol. 15, 2024, ISSN: 2041-1723.

Lin, Jieye; Unge, Johan; Gonen, Tamir
MicroED Structures of Fluticasone Furoate and Fluticasone Propionate Provide New Insights to Their Function
In: bioRxiv, 2024.

Lin, Jieye; Bu, Guanhong; Unge, Johan; Gonen, Tamir
An Updated Structure of Oxybutynin Hydrochloride
In: Advanced Science, 2024, ISSN: 2198-3844.

Clabbers, Max T. B.; Hattne, Johan; Martynowycz, Michael W.; Gonen, Tamir
Energy filtering enables macromolecular MicroED data at sub-atomic resolution
In: bioRxiv, 2024.

Lin, Jieye; Bu, Guanhong; Unge, Johan; Gonen, Tamir
Uncovering the Elusive Structures and Mechanisms of Prevalent Antidepressants
In: Advanced Therapeutics, 2024, ISSN: 2366-3987.

Lin, Jieye; Bu, Guanhong; Unge, Johan; Gonen, Tamir
An Updated Structure of Oxybutynin Hydrochloride
In: bioRxiv, 2024.

Gillman, Cody; Bu, Guanhong; Danelius, Emma; Hattne, Johan; Nannenga, Brent L.; Gonen, Tamir
Eliminating the missing cone challenge through innovative approaches
In: Journal of Structural Biology: X, vol. 9, pp. 100102, 2024, ISSN: 2590-1524.

Nicolas, William J.; Shiriaeva, Anna; Martynowycz, Michael W.; Grey, Angus C; Ruma, Yasmeen; Donaldson, Paul J; Gonen, Tamir
Structure of the lens MP20 mediated adhesive junction
In: bioRxiv, 2024.

Unge, Johan; Lin, Jieye; Weaver, Sara J; Her, Ampon Sae; Gonen, Tamir
Compositional Analysis of Complex Mixtures using Automatic MicroED Data Collection
In: Advanced Science, 2024, ISSN: 2198-3844.

Xia, Xian; Sung, Po-Yu; Martynowycz, Michael W.; Gonen, Tamir; Roy, Polly; Zhou, Z. Hong
RNA genome packaging and capsid assembly of bluetongue virus visualized in host cells
In: Cell, 2024, ISSN: 0092-8674.

Bu, Guanhong; Danelius, Emma; Wieske, Lianne H. E.; Gonen, Tamir
Polymorphic Structure Determination of the Macrocyclic Drug Paritaprevir by MicroED
In: Advanced Biology, vol. 8, iss. 5, pp. 2300570, 2024, ISSN: 2701-0198.

Gillman, Cody; Bu, Guanhong; Danelius, Emma; Hattne, Johan; Nannenga, Brent; Gonen, Tamir
Eliminating the Missing Cone Challenge through Innovative Approaches
In: bioRxiv, 2024.

Ruma, Yasmeen N; Bu, Guanhong; Gonen, Tamir
MicroED structure of the C11 cysteine protease Clostripain
In: bioRxiv, 2024.

Lin, Jieye; Bu, Guanhong; Unge, Johan; Gonen, Tamir
Uncovering the Elusive Structures and Mechanisms of Prevalent Antidepressants
In: bioRxiv, 2024.

2023

Danelius, Emma; Porter, Nicholas J; Unge, Johan; Arnold, Frances H; Gonen, Tamir (Journal Article)
MicroED Structure of a Protoglobin Reactive Carbene Intermediate
In: J Am Chem Soc, vol. 145, no. 13, pp. 7159–7165, 2023, ISSN: 1520-5126.

Danelius, Emma; Patel, Khushboo; Gonzalez, Brenda; Gonen, Tamir (Journal Article)
MicroED in drug discovery
In: Curr Opin Struct Biol, vol. 79, pp. 102549, 2023, ISSN: 1879-033X.

Gillman, Cody; Patel, Khushboo; Unge, Johan; Gonen, Tamir
The structure of the neurotoxin palytoxin determined by MicroED (Bachelor Thesis)
2023.

Gillman, Cody; Nicolas, William J; Martynowycz, Michael W; Gonen, Tamir
Design and implementation of suspended drop crystallization (Bachelor Thesis)
2023.

Martynowycz, Michael W; Shiriaeva, Anna; Clabbers, Max T B; Nicolas, William J; Weaver, Sara J; Hattne, Johan; Gonen, Tamir
A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals (Journal Article)
In: Nat Commun, vol. 14, no. 1, pp. 1086, 2023, ISSN: 2041-1723.

2022

Clabbers, Max T B; Martynowycz, Michael W; Hattne, Johan; Nannenga, Brent L; Gonen, Tamir
Electron-counting MicroED data with the K2 and K3 direct electron detectors (Journal Article)
In: J Struct Biol, vol. 214, no. 4, pp. 107886, 2022, ISSN: 1095-8657.

Clabbers, Max T. B.; Martynowycz, Michael W.; Hattne, Johan; Gonen, Tamir
Hydrogens and hydrogen-bond networks in macromolecular MicroED data (Journal Article)
In: J Struct Biol X, vol. 6, pp. 100078, 2022.

Martynowycz, Michael W.; Shiriaeva, Anna; Clabbers, Max T. B.; Nicolas, William J.; Weaver, Sara J.; Hattne, Johan; Gonen, Tamir
A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals (Online)
2022, visited: 26.07.2022.

Martynowycz, Michael W; Clabbers, Max T B; Hattne, Johan; Gonen, Tamir
Ab initio phasing macromolecular structures using electron-counted MicroED data (Journal Article)
In: Nat Methods, vol. 19, no. 6, pp. 724–729, 2022, ISSN: 1548-7105.

Martynowycz, Mike; Gonen, Tamir
Unlocking the potential of microcrystal electron diffraction (Journal Article)
In: Phys Today, vol. 75, no. 6, pp. 38–42, 2022, ISSN: 0031-9228.

2021

Martynowycz, Michael W; Gonen, Tamir
Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED) (Journal Article)
In: STAR Protoc, vol. 2, no. 3, pp. 100686, 2021, ISSN: 2666-1667.

Danelius, Emma; Gonen, Tamir
Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED (Journal Article)
In: Methods Mol Biol, vol. 2305, pp. 323–342, 2021, ISSN: 1940-6029.

Martynowycz, Michael W; Gonen, Tamir
Studying Membrane Protein Structures by MicroED (Journal Article)
In: Methods Mol Biol, vol. 2302, pp. 137–151, 2021, ISSN: 1940-6029.

Mu, Xuelang; Gillman, Cody; Nguyen, Chi; Gonen, Tamir
An Overview of Microcrystal Electron Diffraction (MicroED) (Journal Article)
In: Annu Rev Biochem, vol. 90, pp. 431–450, 2021, ISSN: 1545-4509.

Martynowycz, Michael W; Gonen, Tamir
Ligand Incorporation into Protein Microcrystals for MicroED by On-Grid Soaking (Journal Article)
In: Structure, vol. 29, no. 1, pp. 88–95.e2, 2021, ISSN: 1878-4186.

Martynowycz, Michael W; Gonen, Tamir
Microcrystal Electron Diffraction of Small Molecules (Journal Article)
In: J Vis Exp, no. 169, 2021, ISSN: 1940-087X.

Danelius, Emma; Halaby, Steve; van der Donk, Wilfred A; Gonen, Tamir
MicroED in natural product and small molecule research (Journal Article)
In: Nat Prod Rep, vol. 38, no. 3, pp. 423–431, 2021, ISSN: 1460-4752.

Martynowycz, Michael W; Shiriaeva, Anna; Ge, Xuanrui; Hattne, Johan; Nannenga, Brent L; Cherezov, Vadim; Gonen, Tamir
MicroED structure of the human adenosine receptor determined from a single nanocrystal in LCP (Journal Article)
In: Proc Natl Acad Sci U S A, vol. 118, no. 36, 2021, ISSN: 1091-6490.

Martynowycz, Michael W; Clabbers, Max T B; Unge, Johan; Hattne, Johan; Gonen, Tamir
Benchmarking the ideal sample thickness in cryo-EM (Journal Article)
In: Proc Natl Acad Sci U S A, vol. 118, no. 49, 2021, ISSN: 1091-6490.

Lei, Hsiang-Ting; Mu, Xuelang; Hattne, Johan; Gonen, Tamir
A conformational change in the N terminus of SLC38A9 signals mTORC1 activation (Journal Article)
In: Structure, vol. 29, no. 5, pp. 426–432.e8, 2021, ISSN: 1878-4186.

2020

Richards, Logan S; Millán, Claudia; Miao, Jennifer; Martynowycz, Michael W; Sawaya, Michael R; Gonen, Tamir; Borges, Rafael J; Usón, Isabel; Rodriguez, Jose A
Fragment-based determination of a proteinase K structure from MicroED data using ARCIMBOLDO_SHREDDER (Journal Article)
In: Acta Crystallogr D Struct Biol, vol. 76, no. Pt 8, pp. 703–712, 2020, ISSN: 2059-7983.

Nguyen, Chi; Gonen, Tamir
Beyond protein structure determination with MicroED (Journal Article)
In: Curr Opin Struct Biol, vol. 64, pp. 51–58, 2020, ISSN: 1879-033X.

Martynowycz, Michael W; Hattne, Johan; Gonen, Tamir
Experimental Phasing of MicroED Data Using Radiation Damage (Journal Article)
In: Structure, vol. 28, no. 4, pp. 458–464.e2, 2020, ISSN: 1878-4186.

Martynowycz, Michael W; Khan, Farha; Hattne, Johan; Abramson, Jeff; Gonen, Tamir
MicroED structure of lipid-embedded mammalian mitochondrial voltage-dependent anion channel (Journal Article)
In: Proc Natl Acad Sci U S A, vol. 117, no. 51, pp. 32380–32385, 2020, ISSN: 1091-6490.

Zhu, Lan; Bu, Guanhong; Jing, Liang; Shi, Dan; Lee, Ming-Yue; Gonen, Tamir; Liu, Wei; Nannenga, Brent L
Structure Determination from Lipidic Cubic Phase Embedded Microcrystals by MicroED (Journal Article)
In: Structure, vol. 28, no. 10, pp. 1149–1159.e4, 2020, ISSN: 1878-4186.