Publications

Referencing us

MEDIC must be acknowledged in all publications that resulted from either collaboration with the Center or with individual personnel of the Center or utilized services of the Center. The NIH grant funding the MEDIC project is NIH/GM P41 136508.

As in all scientific publications, co-authorship for a member of the Scientific Staff is warranted when an individual has made a significant contribution to the scientific work being described.

Data policy

All data collected for structural purposes will be made publicly available upon publication. Structures will be deposited in the protein structure data bank (PDB) and the electron microscopy data bank (EMDB) prior to publication. All primary data associated with a structure will be made available to the community upon request.

Sharing of materials

All materials, plasmids, peptides, and protein fragments will be made available to the research community in accordance with NIH guidelines and in coordination with the UCLA technology development group (TDG) governing material transfer agreements (MTA).

Sharing of software and algorithms

All software and programs used for analysis or structure determination will be made available either as pseudocode or as standalone scripts/programs in accordance with NIH guidelines and in coordination with the UCLA technology development group (TDG) governing intellectual property dissemination and materials transfer agreements (MTA).

Publication list

2023

Danelius, Emma; Porter, Nicholas J; Unge, Johan; Arnold, Frances H; Gonen, Tamir (Journal Article)
MicroED Structure of a Protoglobin Reactive Carbene Intermediate
In: J Am Chem Soc, vol. 145, no. 13, pp. 7159–7165, 2023, ISSN: 1520-5126.

Danelius, Emma; Patel, Khushboo; Gonzalez, Brenda; Gonen, Tamir (Journal Article)
MicroED in drug discovery
In: Curr Opin Struct Biol, vol. 79, pp. 102549, 2023, ISSN: 1879-033X.

Gillman, Cody; Patel, Khushboo; Unge, Johan; Gonen, Tamir 
The structure of the neurotoxin palytoxin determined by MicroED (Bachelor Thesis)
2023. 

Gillman, Cody; Nicolas, William J; Martynowycz, Michael W; Gonen, Tamir
Design and implementation of suspended drop crystallization (Bachelor Thesis)
2023.

Martynowycz, Michael W; Shiriaeva, Anna; Clabbers, Max T B; Nicolas, William J; Weaver, Sara J; Hattne, Johan; Gonen, Tamir
A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals (Journal Article)
In: Nat Commun, vol. 14, no. 1, pp. 1086, 2023, ISSN: 2041-1723.

2022

Clabbers, Max T B; Martynowycz, Michael W; Hattne, Johan; Nannenga, Brent L; Gonen, Tamir
Electron-counting MicroED data with the K2 and K3 direct electron detectors (Journal Article)
In: J Struct Biol, vol. 214, no. 4, pp. 107886, 2022, ISSN: 1095-8657.

Clabbers, Max T. B.; Martynowycz, Michael W.; Hattne, Johan; Gonen, Tamir
Hydrogens and hydrogen-bond networks in macromolecular MicroED data (Journal Article)
In: J Struct Biol X, vol. 6, pp. 100078, 2022.

Martynowycz, Michael W.; Shiriaeva, Anna; Clabbers, Max T. B.; Nicolas, William J.; Weaver, Sara J.; Hattne, Johan; Gonen, Tamir
A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals (Online)
2022, visited: 26.07.2022.

Martynowycz, Michael W; Clabbers, Max T B; Hattne, Johan; Gonen, Tamir
Ab initio phasing macromolecular structures using electron-counted MicroED data (Journal Article)
In: Nat Methods, vol. 19, no. 6, pp. 724–729, 2022, ISSN: 1548-7105.

Martynowycz, Mike; Gonen, Tamir
Unlocking the potential of microcrystal electron diffraction (Journal Article)
In: Phys Today, vol. 75, no. 6, pp. 38–42, 2022, ISSN: 0031-9228.

2021

Martynowycz, Michael W; Gonen, Tamir
Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED) (Journal Article)
In: STAR Protoc, vol. 2, no. 3, pp. 100686, 2021, ISSN: 2666-1667.

Danelius, Emma; Gonen, Tamir
Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED (Journal Article)
In: Methods Mol Biol, vol. 2305, pp. 323–342, 2021, ISSN: 1940-6029.

Martynowycz, Michael W; Gonen, Tamir
Studying Membrane Protein Structures by MicroED (Journal Article)
In: Methods Mol Biol, vol. 2302, pp. 137–151, 2021, ISSN: 1940-6029.

Mu, Xuelang; Gillman, Cody; Nguyen, Chi; Gonen, Tamir
An Overview of Microcrystal Electron Diffraction (MicroED) (Journal Article)
In: Annu Rev Biochem, vol. 90, pp. 431–450, 2021, ISSN: 1545-4509.

Martynowycz, Michael W; Gonen, Tamir
Ligand Incorporation into Protein Microcrystals for MicroED by On-Grid Soaking (Journal Article)
In: Structure, vol. 29, no. 1, pp. 88–95.e2, 2021, ISSN: 1878-4186.

Martynowycz, Michael W; Gonen, Tamir
Microcrystal Electron Diffraction of Small Molecules (Journal Article)
In: J Vis Exp, no. 169, 2021, ISSN: 1940-087X.

Danelius, Emma; Halaby, Steve; van der Donk, Wilfred A; Gonen, Tamir
MicroED in natural product and small molecule research (Journal Article)
In: Nat Prod Rep, vol. 38, no. 3, pp. 423–431, 2021, ISSN: 1460-4752.

Martynowycz, Michael W; Shiriaeva, Anna; Ge, Xuanrui; Hattne, Johan; Nannenga, Brent L; Cherezov, Vadim; Gonen, Tamir
MicroED structure of the human adenosine receptor determined from a single nanocrystal in LCP (Journal Article)
In: Proc Natl Acad Sci U S A, vol. 118, no. 36, 2021, ISSN: 1091-6490.

Martynowycz, Michael W; Clabbers, Max T B; Unge, Johan; Hattne, Johan; Gonen, Tamir
Benchmarking the ideal sample thickness in cryo-EM (Journal Article)
In: Proc Natl Acad Sci U S A, vol. 118, no. 49, 2021, ISSN: 1091-6490.

Lei, Hsiang-Ting; Mu, Xuelang; Hattne, Johan; Gonen, Tamir
A conformational change in the N terminus of SLC38A9 signals mTORC1 activation (Journal Article)
In: Structure, vol. 29, no. 5, pp. 426–432.e8, 2021, ISSN: 1878-4186.

2020

Richards, Logan S; Millán, Claudia; Miao, Jennifer; Martynowycz, Michael W; Sawaya, Michael R; Gonen, Tamir; Borges, Rafael J; Usón, Isabel; Rodriguez, Jose A
Fragment-based determination of a proteinase K structure from MicroED data using ARCIMBOLDO_SHREDDER (Journal Article)
In: Acta Crystallogr D Struct Biol, vol. 76, no. Pt 8, pp. 703–712, 2020, ISSN: 2059-7983.

Nguyen, Chi; Gonen, Tamir
Beyond protein structure determination with MicroED (Journal Article)
In: Curr Opin Struct Biol, vol. 64, pp. 51–58, 2020, ISSN: 1879-033X.

Martynowycz, Michael W; Hattne, Johan; Gonen, Tamir
Experimental Phasing of MicroED Data Using Radiation Damage (Journal Article)
In: Structure, vol. 28, no. 4, pp. 458–464.e2, 2020, ISSN: 1878-4186.

Martynowycz, Michael W; Khan, Farha; Hattne, Johan; Abramson, Jeff; Gonen, Tamir
MicroED structure of lipid-embedded mammalian mitochondrial voltage-dependent anion channel (Journal Article)
In: Proc Natl Acad Sci U S A, vol. 117, no. 51, pp. 32380–32385, 2020, ISSN: 1091-6490.

Zhu, Lan; Bu, Guanhong; Jing, Liang; Shi, Dan; Lee, Ming-Yue; Gonen, Tamir; Liu, Wei; Nannenga, Brent L
Structure Determination from Lipidic Cubic Phase Embedded Microcrystals by MicroED (Journal Article)
In: Structure, vol. 28, no. 10, pp. 1149–1159.e4, 2020, ISSN: 1878-4186.