Design and implementation of suspended drop crystallization Cody Gillman, William J Nicolas, Michael W Martynowycz, Tamir Gonen We have developed a novel crystal growth method known as suspended drop crystallization. Unlike traditional methods,…
The structure of the neurotoxin palytoxin determined by MicroED Cody Gillman, Khushboo Patel, Johan Unge, Tamir Gonen Palytoxin (PTX) is a potent neurotoxin found in marine animals that can cause serious symptoms such…
MicroED Structure of a Protoglobin Reactive Carbene Intermediate Emma Danelius , Nicholas J Porter , Johan Unge , Frances H Arnold , Tamir Gonen Microcrystal electron diffraction (MicroED) is an emerging technique that has shown great potential for…
A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals Michael W Martynowycz , Anna Shiriaeva , Max T B Clabbers, William J Nicolas , Sara J Weaver, Johan Hattne , Tamir Gonen Crystallizing G…
MicroED in drug discovery Emma Danelius Khushboo Patel , Brenda Gonzalez , Tamir Gonen The cryo-electron microscopy (cryo-EM) method microcrystal electron diffraction (MicroED) was initially described in 2013 and has recently gained attention as an…
Unlocking the potential of MICROCRYSTAL ELECTRON DIFFRACTION Mike Martynowycz , Tamir Gonen Atoms stick together in different ways to make the molecules that compose everything we touch and see. Our bodies are…
Benchmarking the ideal sample thickness in cryo-EM Michael W Martynowycz 1 2, Max T B Clabbers 2, Johan Unge 2, Johan Hattne 1 2, Tamir Gonen 3 2 4 The relationship between sample thickness and quality of data obtained is investigated by microcrystal…
#cryoEM #MicroED with electron counting. Protein structure at 0.8A resolution. Ab initio phasing for two proteins facilitated by electron counting on a falcon 4 direct electron detector. A section of…
One of expected areas where MicroED may have an outstanding role is for proteins and complexes where crystal growth tend to yield smaller crystals only. Membrane proteins puts many challenges…